The mobile phase is usually an inert gas or an unreactive gas such as helium, argon, nitrogen or hydrogen. Gas chromatography is the process of separating compounds in a mixture by injecting a gaseous or liquid sample into a mobile phase, typically called the carrier gas, and passing the gas through a stationary phase. If a sample contains salts or carries a charge, it must be analyzed using HPLC, not GC. GC is used for volatile compounds (those that break down rapidly) while HPLC is better for less volatile samples. The FID is sensitive to, and capable of detecting, compounds that contain carbon atoms (C), which accounts for almost all organic compounds. The FID is the most common detector used in gas chromatography.
HPLC uses a solvent for the mobile phase where the polarity, solubility and complexity of the compounds in the sample determine which solvent should be used. The main difference between HPLC and GC is the type of mobile phase used, which is where the distinction in the names come from. What are the main differences between GC and HPLC? 7 What kind of flash lamp is used for Agilent?.6 What is the difference between LC and GC?.5 What is the basic principle of gas chromatography?.1 What are the main differences between GC and HPLC?.
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